Sca-1 Antibody / Ly6A/E

• Description:

  Stem cell antigen-1 (Sca-1) is a mouse glycosyl phosphatidylinositol-anchored protein and a cell surface marker found on hematopoietic stem cells (HSCs) . It is encoded by the strain-specific Ly-6 E/A allelic gene. Sca-1 has been discovered in several non hematopoietic tissues and can be used to enrich progenitor cell populations other than HSC. It is suggested that Sca-1 could be involved in regulating both B and T cell activation.

• Specifications:

  Western blot: 0.5-1 µg/mL

• UniProt:

  P05533

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  Amino acids 48-75 (YPDGVCVTQEAAVIVDSQTRKVKNNLCL) from the mouse protein were used as the immunogen for the Sca-1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

• Reconstitution:

  After reconstitution, the Sca-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Sca-1 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Sca-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Sca-1 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of mouse HepA1-6 cell lysate with Sca-1 antibody at 0.5ug/ml. Expected molecular weight: 14-18 kDa.