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NEDD8 Antibody

CAT: 0800-R32590Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32590Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NEDD8 is a protein that in humans is encoded by the NEDD8 gene. Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The most substrates of NEDD8 modification are the Cullin subunits of Cullin-based E3 ubiquitin ligases, which are active only when neddylated. Their NEDDylation is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Immunofluorescence (FFPE) : 2-4 µg/mL
Gene ID
4738
UniProt
Q15843
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 20-60 (TDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYK-human) were used as the immunogen for the NEDD8 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the NEDD8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This NEDD8 antibody is available for research use only.
Storage Conditions
After reconstitution, the NEDD8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the NEDD8 antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic
Image Legend
Immunofluorescent staining of FFPE human A431 cells with NEDD8 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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