Cytokeratin 5 Antibody

• Description:

  Cytokeratin 5, also known as Keratin 5, KRT5, K5, or CK5, is a protein that is encoded in humans by the KRT5 gene. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

• Specifications:

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

• Gene ID:

  3852

• UniProt:

  P13647

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Amino acids 286-317 (KVELEAKVDALMDEINFMKMFFDAELSQMQTH-human) were used as the immunogen for the Cytokeratin 5 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the Cytokeratin 5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Cytokeratin 5 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Cytokeratin 5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Cytokeratin 5 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  IHC testing of FFPE human tonsil tissue with Cytokeratin 5 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.