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HPRT Antibody

CAT: 0800-R32539Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32539Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) is an enzyme encoded in humans by the HPRT1 gene. The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
P00492
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids A2-S110 from the human protein were used as the immunogen for the HPRT antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the HPRT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This HPRT antibody is available for research use only.
Storage Conditions
After reconstitution, the HPRT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Differences in protocols and secondary/substrate sensitivity may require the HPRT antibody to be titrated for optimal performance.
Location
Cytoplasmic
Image Legend
Western blot testing of 1) rat brain, 2) mouse brain and 3) human HeLa lysate with HPRT antibody at 0.5ug/ml. Predicted/observed molecular weight: ~24 kDa.

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