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EpCAM Antibody

CAT: 0800-R32527Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32527Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/10^6 cells, ELISA: 0.5-1 µg/mL (human protein tested)
UniProt
P16422
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids 147-189 (ELKHKAREKPYDSKSLRTALQKEITTRYQLDPKFITSILYENN) from the human protein were used as the immunogen for the EpCAM antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS, ELISA (protein)
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the EpCAM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This EpCAM antibody is available for research use only.
Storage Conditions
After reconstitution, the EpCAM antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Differences in protocols and secondary/substrate sensitivity may require the EpCAM antibody to be titrated for optimal performance.
Location
Cell surface, cytoplasmic
Image Legend
Immunofluorescent staining of human rectal cancer tissue with EpCAM antibody (red) and DAPI (blue) . HIER: steam sections in pH6 citrate buffer for 20 min.

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