CD40 Antibody

• Description:

  CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. It is a member of the tumor necrosis factor (TNF) receptor superfamily and is the receptor for CD40 ligand.

• Specifications:

  Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, FACS: 1-3ug/10^6 cells, ELISA: 0.5-1 µg/mL (mouse protein tested) ; request BSA-free format for coating

• UniProt:

  P27512

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Amino acids L20-R193 from the mouse protein were used as the immunogen for the CD40 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, FACS, ELISA (protein)

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

• Reconstitution:

  After reconstitution, the CD40 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This CD40 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the CD40 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Differences in protocols and secondary/substrate sensitivity may require the CD40 antibody to be titrated for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, cell membrane

• Image Legend:

  Western blot testing of mouse spleen lysate with CD40 antibody at 0.5ug/ml. Predicted molecular weight is 32-45 kDa depending on glycosylation level.