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CD40 Antibody

CAT: 0800-R32519Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32519Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. It is a member of the tumor necrosis factor (TNF) receptor superfamily and is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.5-1 µg/mL (human protein tested) ; request BSA-free format for coating
UniProt
P25942
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids E21-R193 from the human protein were used as the immunogen for the CD40 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, ELISA (protein)
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the CD40 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CD40 antibody is available for research use only.
Storage Conditions
After reconstitution, the CD40 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Differences in protocols and secondary/substrate sensitivity may require the CD40 antibody to be titrated for optimal performance.
Location
Cytoplasmic, cell membrane
Image Legend
Flow cytometry testing of human PBM cells with CD40 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= CD40 antibody.

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