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ARHGEF1 Antibody

CAT: 0800-R32513Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32513Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Rho guanine nucleotide exchange factor 1, also called p115-RhoGEF, is a protein that in humans is encoded by the ARHGEF1 gene. Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
Q92888
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 41-71 (EQNSQFQSLEQVKRRPAHLMALLQHVALQFE) from the human protein were used as the immunogen for the ARHGEF1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the ARHGEF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ARHGEF1 antibody is available for research use only.
Storage Conditions
After reconstitution, the ARHGEF1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Differences in protocols and secondary/substrate sensitivity may require the ARHGEF1 antibody to be titrated for optimal performance.
Location
Cytoplasmic, membranous
Image Legend
Immunofluorescent staining of FFPE human A431 cells with ARHGEF1 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · Q92888

Rho guanine nucleotide exchange factor 1

ARHG1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q92888
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ARHGEF1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
O00513, Q6NX52, Q8N4J4, Q96BF4, Q96F17, Q9BSB1
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Domain

Coiled coil

Cellular component

CytoplasmMembrane

Molecular function

GTPase activationGuanine-nucleotide releasing factor

PTM

Phosphoprotein

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