ABHD5 Antibody / CGI-58

• Description :

  1-acylglycerol-3-phosphate O-acyltransferase ABHD5 is an enzyme that in humans is encoded by the ABHD5 gene. The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated withChanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.

• CAS Number :

  9007-83-4

• Specifications :

  Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, IF/ICC (FFPE) : 2-4 µg/mL

• UniProt :

  Q8WTS1

• Host :

  Rabbit

• Reactivity :

  Human, Rat

• Immunogen :

  Amino acids R169-D349 from the human protein were used as the immunogen for the ABHD5 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, IHC-P, IF/ICC

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the ABHD5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This ABHD5 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the ABHD5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Differences in protocols and secondary/substrate sensitivity may require the ABHD5 antibody to be titrated for optimal performance.

• Location :

  Cytoplasmic

• Image Legend :

  Western blot testing of 1) rat kidney and 2) human A431 lysate with ABHD5 antibody at 0.5ug/ml. Predicted molecular weight ~39 kDa.