ADH5 Antibody

CAT: 0800-R32485Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-R32485Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Alcohol dehydrogenase class-3, also called Alcohol dehydrogenase 5, is an enzyme that in humans is encoded by the ADH5 gene. This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL
UniProt
P11766
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids K212-I374 from the human protein were used as the immunogen for the ADH5 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the ADH5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ADH5 antibody is available for research use only.
Storage Conditions
After reconstitution, the ADH5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Differences in protocols and secondary/substrate sensitivity may require the ADH5 antibody to be titrated for optimal performance.
Location
Cytoplasmic
Image Legend
Immunofluorescent staining of FFPE human A431 cells with ADH5 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

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