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Visfatin Antibody

CAT: 0800-R32446Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32446Size:100 µg
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Description
Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin, is an enzyme that in humans is encoded by the PBEF1 gene. This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, ELISA: 0.1-0.5 µg/mL (human protein tested) ; request BSA-free format for coating
UniProt
P43490
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids L62-N337 were used as the immunogen for the Visfatin antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ELISA (protein)
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
Prior to reconstitution, store at 4oC. After reconstitution, the Visfatin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Visfatin antibody is available for research use only.
Storage Conditions
Prior to reconstitution, store at 4°C. After reconstitution, the Visfatin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Visfatin antibody should be determined by the researcher.
Location
Predominantly cytoplasmic, some nuclear staining may also be seen
Image Legend
Western blot testing of 1) rat brain, 2) mouse heart and 3) human placenta lysate with Visfatin antibody at 0.5ug/ml. Expected molecular weight ~56 kDa.

UniProtKB · P43490

Nicotinamide phosphoribosyltransferase

NAMPT_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P43490
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NAMPT
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
NAmPRTase, Nampt
EC number
2.4.2.12
Processing
—
Secondary accessions
A4D0Q9, A4D0R0, Q3KQV0, Q8WW95
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Biological process

Biological rhythmsPyridine nucleotide biosynthesis

Molecular function

CytokineGlycosyltransferaseTransferase

Cellular component

CytoplasmNucleusSecreted

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