CDCP1 Antibody / SIMA135

CAT: 0800-R32422Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-R32422Size:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CUB domain-containing protein 1 (CDCP1) is a protein that in humans is encoded by the CDCP1 gene. It has also been designated as CD318 (cluster of differentiation 318) and Trask (Transmembrane and associated with src kinases) . CDCP1/Trask is a 140 kD transmembrane glycoprotein with a large extracellular domain (ECD) containing two CUB domains, and a smaller intracellular domain (ICD) containing five tyrosines. The tyrosine phosphorylation of Trask is tightly regulated and reciprocally linked with the state of cell adhesion. The tyrosine phosphorylation of CDCP1 in cultured cells occurs when cells are induced to detach by trypsin or EDTA, or seen spontaneously during mitotic detachment. The overexpression of CDCP1 leads to the loss of cell adhesion and a detached phenotype. CDCP1 is widely expressed in human epithelial tissues, but its phosphorylation is only seen in mitotically detached or shedding cells, consistent with its role in the negative regulation of cell adhesion.
CAS Number
9007-83-4
Specifications
Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells
Gene ID
64866
UniProt
Q9H5V8
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids R582-T667 of the human protein were used as the immunogen for the CDCP1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the CDCP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CDCP1 antibody is available for research use only.
Storage Conditions
After reconstitution, the CDCP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the CDCP1 antibody should be determined by the researcher.1. Required HIER for FFPE samples: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
Location
Cytoplasmic, membranous
Image Legend
Western blot testing of human SW620 cell lysate with CDCP1 antibody. Expected molecular weight: 93 kDa (unmodified), 130-140 kDa (glycosylated), ~70 kDa (cleaved) .
Product Datasheet
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