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ZAP70 Antibody

CAT: 0800-R32411Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32411Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ZAP-70 (Zeta-chain-associated protein kinase 70) encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
CAS Number
9007-83-4
Specifications
Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL
Gene ID
7535
UniProt
P43403
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids MRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQL from the human protein were used as the immunogen for the ZAP70 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the ZAP70 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ZAP70 antibody is available for research use only.
Storage Conditions
After reconstitution, the ZAP70 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the ZAP70 antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Western blot testing of human 1) Jurkat and 2) CEM cell lysate with ZAP70 antibody. Expected molecular weight ~70 kDa.

UniProtKB · P43403

Tyrosine-protein kinase ZAP-70

ZAP70_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P43403
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ZAP70
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.7.10.2
Processing
—
Secondary accessions
A6NFP4, Q6PIA4, Q8IXD6, Q9UBS6
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Biological process

Adaptive immunityImmunity

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Cellular component

Cell membraneCytoplasmMembrane

Disease

Disease variantSCID

Molecular function

KinaseTransferaseTyrosine-protein kinase

Domain

RepeatSH2 domain

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