DDB2 Antibody

• Description :

  DNA damage-binding protein 2 is a protein that in humans is encoded by the DDB2 gene. This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. And this subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene.

• CAS Number :

  9007-83-4

• Specifications :

  Western blot: 0.1-0.5 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt :

  Q92466

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  Amino acids 1-115 of human DDB2 were used as the immunogen for the DDB2 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, IF, FACS

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the DDB2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This DDB2 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the DDB2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the DDB2 antibody should be determined by the researcher.

• Image Legend :

  Immunofluorescent staining of FFPE human U-2 OS cells with DDB2 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.