MAFA Antibody

• Description:

  MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene. MAFA plays a key role in coordinating and controlling the level of insulin gene expression in islet beta cells. It is located in 8q24.3. The intracellular domain of MAFA contains a single immunoreceptor tyrosine-based inhibitory motif (ITIM), which classifies MAFA as a member of an inhibitory receptor superfamily. The inhibitory capacity of MAFA is best defined in mast cells, where MAFA keeps in check the antigen-induced (i.e. Fc epsilon RI-induced) secretion ofinflammatory mediators.

• Specifications:

  Western blot: 0.1-0.5 µg/mL

• UniProt:

  Q8NHW3

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  Amino acids EDAVEALIGSGHHGAHHGAHHPAAAAAYEAFR of human MAFA were used as the immunogen for the MAFA antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the MAFA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This MAFA antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the MAFA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the MAFA antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of human 1) HEK293, 2) K562, 3) 22RV1 and 4) rat PC-12 lysate with MAFA antibody. Predicted molecular weight ~37 kDa.