MAFA Antibody

• Description :

  MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene. MAFA plays a key role in coordinating and controlling the level of insulin gene expression in islet beta cells. It is located in 8q24.3. The intracellular domain of MAFA contains a single immunoreceptor tyrosine-based inhibitory motif (ITIM), which classifies MAFA as a member of an inhibitory receptor superfamily. The inhibitory capacity of MAFA is best defined in mast cells, where MAFA keeps in check the antigen-induced (i.e. Fc epsilon RI-induced) secretion ofinflammatory mediators.

• Specifications :

  Western blot: 0.1-0.5 µg/mL

• UniProt :

  Q8NHW3

• Host :

  Rabbit

• Reactivity :

  Human, Rat

• Immunogen :

  Amino acids EDAVEALIGSGHHGAHHGAHHPAAAAAYEAFR of human MAFA were used as the immunogen for the MAFA antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the MAFA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This MAFA antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the MAFA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the MAFA antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot testing of human 1) HEK293, 2) K562, 3) 22RV1 and 4) rat PC-12 lysate with MAFA antibody. Predicted molecular weight ~37 kDa.