Calcineurin A Antibody / PPP3CA

• Description:

  Calcineurin A is also known as PPP3CA. It is mapped to 4q24. Semsarian et al. (1999) and Musaro et al. (1999) independently showed that IGF1stimulates skeletal muscle hypertrophy and a switch to glycolytic metabolism by activating calcineurin A and inducing the nuclear translocation of transcription factor NFATC1. Semsarian et al. (1999) found that hypertrophy was suppressed by the calcineurin inhibitors cyclosporin A or FK506, but not by inhibitors of the MAP kinase or phosphatidylinositol-3-OH kinase pathways. Musaro et al. (1999) showed that expression of a dominant-negative calcineurin mutant also repressed myocyte differentiation and hypertrophy. Musaro et al. (1999) demonstrated that either IGF1 or activated calcineurin induces expression of transcription factor GATA2, which accumulates in a subset of myocyte nuclei, where it associates with calcineurin and a specific dephosphorylated isoform of NFATC1.

• Specifications:

  Western blot: 0.1-0.5 µg/mL

• UniProt:

  Q08209

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Amino acids DAMPSDANLNSINKALTSETNGTDSNGSNSSNIQ of human Calcineurin A were used as the immunogen for the Calcineurin A antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the Calcineurin A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Calcineurin A antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Calcineurin A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Calcineurin A antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of human 1) HeLa, 2) A549 and 3) COLO320 lysate with Calcineurin A antibody. Predicted molecular weight ~61 kDa, observed here at ~84 kDa.