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SF2 Antibody / SRSF1

CAT: 0800-R32198Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32198Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SF2, also known as Serine/arginine-rich splicing factor 1 (SRSF1), is a protein that in humans is encoded by the SFRS1 gene. This gene encodes a member of the arginine/serine-rich splicing factor protein family. There is a pseudogene of this gene on chromosome 13. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. ASF/SF2 is necessary for all splicing reactions to occur, and influences splice site selection in a concentration-dependent manner, resulting in alternative splicing.
CAS Number
9007-83-4
Specifications
Western blot: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL
UniProt
Q07955
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids VIRGPAGNNDCRIYVGNLPPDIRTKDIE of human SF2 were used as the immunogen for the SF2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the SF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This SF2 antibody is available for research use only.
Storage Conditions
After reconstitution, the SF2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the SF2 antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic
Image Legend
IHC testing of FFPE human placental tissue with SF2 antibody. HIER: Boil the paraffin sections in pH8 EDTA for 20 minutes and allow to cool prior to staining.

UniProtKB · Q07955

Serine/arginine-rich splicing factor 1

SRSF1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q07955
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SRSF1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B2R6Z7, D3DTZ3, Q13809
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondMethylationPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Cellular component

ChromosomeCytoplasmNucleusSpliceosome

Disease

Disease variantIntellectual disability

Biological process

mRNA processingmRNA splicingmRNA transportTransport

Domain

Repeat

Molecular function

RNA-binding

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