Products for Research Use Only
RegisterLogin

ZP2 Antibody

CAT: 0800-R32192Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-R32192Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Zona pellucida sperm-binding protein 2 is a protein that in humans is encoded by the ZP2 gene. The sperm-binding domain on the ZP2 protein is necessary in both humans and mice for oocyte-sperm recognition and penetration of the zona pellucida. It is also responsible for the primary block to polyspermy in mammals. The oocyte has cortical granules peripherally located under the cortex that contain a proteolytic protein called ovastacin. After the sperm binds to ZP2, the cortical granules are exocytosed releasing ovastacin into the perivitelline space. Ovastacin cleaves ZP2 at the N terminus, preventing more sperm from binding and penetrating the oocyte, thus hardening the zona pellucida. Ovastacin is only found in oocytes, and is part of the astacin family of metalloendoproteases. Female mice engineered without ovastacin showed that ZP2 was not cleaved after fertilization.
CAS Number
9007-83-4
Specifications
Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL
UniProt
Q05996
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids ENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDD of human ZP2 were used as the immunogen for the ZP2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IHC-F
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Reconstitution
After reconstitution, the ZP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ZP2 antibody is available for research use only.
Storage Conditions
After reconstitution, the ZP2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the ZP2 antibody should be determined by the researcher.
Location
Cytoplasmic, membrane, secreted
Image Legend
Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with ZP2 antibody. Expected/observed molecular weight ~82 kDa.

Related Products

CatalogName
RQ4624NONO Antibody / p54nrb Antibody
F53954-0.05MLAntibody
F53954-0.2MLAntibody
F53952-0.2MLAntibody
F53952-0.05MLAntibody
V8674-20UGInhibin alpha Antibody / INHA antibody
V8674SAF-100UGInhibin alpha Antibody / INHA antibody
V8674-100UGInhibin alpha Antibody / INHA antibody
R20161SAF-250UGLambda Light Chain Antibody / Lambda Antibody
R30971B-Actin Antibody / Beta Actin Antibody

Alternative Products

CatalogName
MBS8529199-01H2B Antibody Antibody
MBS8513599-01CD11b Antibody Antibody
MBS850197-01Antibody to IGFBP2 Antibody
DF8746-01ASAP1 antibody Antibody
E36403ZNF98 Antibody Antibody
GWB-250026Lck antibody Antibody
E8M1007-4HSP60 Antibody Antibody
MBS9403836-01SZT2 Antibody Antibody
LSMab09882anti-Antibody^Polyclonal antibody control antibody
GWB-5C6547MAD1L1 antibody [9B10] Antibody

Popular Products