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MtTFA Antibody / TFAM

CAT: 0800-R32176Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32176Size:100 µg
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Description
TFAM (Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a protein that activates transcription of each mitochondrial DNA (mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al. (1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott (2007) stated that the sequences mapped to chromosomes 7p (TCF6L1) and 11q (MTTF1, or TCF6L3) are pseudogenes. Larsson et al. (1997) mapped the mouse mitochondrial transcription factor A gene (Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Immunoprecipitation: 2ug per 500ug of lysate
UniProt
Q00059
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids EMKSWEEQMIEVGRKDLLRRTIKKQRKY of human mtTFA were used as the immunogen for the mtTFA antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, IP
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the mtTFA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This mtTFA antibody is available for research use only.
Storage Conditions
After reconstitution, the mtTFA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the mtTFA antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IF/ICC staining of FFPE human A431 cells with mtTFA antibody (green) at 2ug/ml and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · Q00059

Transcription factor A, mitochondrial

TFAM_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q00059
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TFAM
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
mtTFA
EC number
—
Processing
Precursor
Secondary accessions
A8MRB2, A9QXC6, B5BU05, Q5U0C6
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Molecular function

ActivatorDNA-binding

Coding sequence diversity

Alternative splicing

Disease

Disease variantPrimary mitochondrial disease

Cellular component

MitochondrionMitochondrion nucleoid

PTM

Phosphoprotein

Domain

RepeatTransit peptide

Biological process

TranscriptionTranscription regulation

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