STAT5b Antibody

• Description:

  STAT5B (Signal transducer and activator of transcription 5B) is a protein that in humans is encoded by the STAT5B gene. STAT5B orthologs have been identified in most placentals for which complete genome data are available. By FISH, Lin et al. (1996) mapped STAT5B to 17q11.2. The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APML) . The dysregulation of the signaling pathways mediated by this protein may be the cause of the APML.

• Specifications:

  Western blot: 0.1-0.5 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt:

  P51692

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  Amino acids KDEVYSKYYTPVPCESATAKAVDGYVKPQ of human STAT5b were used as the immunogen for the STAT5b antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IF, FACS

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the STAT5b antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This STAT5b antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the STAT5b antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the STAT5b antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Immunofluorescent staining of human U-2 OS cells with STAT5b antibody (red) and DAPI nuclear stain (blue) .