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PPT1 Antibody

CAT: 0800-R32123Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32123Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Palmitoyl-protein thioesterase 1 (PPT-1), also known as palmitoyl-protein hydrolase 1, is an enzyme that in humans is encoded by the PPT1 gene. PPT-1 is a member of the palmitoyl protein thioesterase family. The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4) . Two transcript variants encoding different isoforms have been found for this gene.
CAS Number
9007-83-4
Specifications
Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P50897
Host
Rabbit
Reactivity
Human, Rat
Immunogen
Amino acids KEDVYRNHSIFLADINQERGINESYKKNLMALKK of human PPT1 were used as the immunogen for the PPT1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the PPT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PPT1 antibody is available for research use only.
Storage Conditions
After reconstitution, the PPT1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the PPT1 antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Western blot testing of 1) rat brain, 2) mouse brain, 3) rat liver, 4) mouse liver, 5) human HepG2, 6) human 293T and 7) MCF-7 lysate with PPT1 antibody. Expected/observed molecular weight ~34 kDa.

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