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HINT1 Antibody

CAT: 0800-R32118Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32118Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Histidine triad nucleotide-binding protein 1 is a protein that in humans is encoded by the HINT1 gene. It is a haploinsufficient tumor suppressor gene that inhibits the Wnt/b-catenin pathway in colon cancer cells and Microphthalmia-associated transcription factor (MITF) activity in human mast cells. The protein encoded by this gene can hydrolyze substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate. Several transcript variants, but only one of them protein-coding, have been found for this gene.
CAS Number
9007-83-4
Specifications
Western blot: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P49773
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids HISQISVAEDDDESLLGHLMIVGKKCAADLGLNK of human HINT1 were used as the immunogen for the HINT1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the HINT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This HINT1 antibody is available for research use only.
Storage Conditions
After reconstitution, the HINT1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the HINT1 antibody should be determined by the researcher.
Location
Cytoplasmic, nuclear
Image Legend
Immunofluorescent staining of FFPE human A431 cells with HINT1 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · P49773

Adenosine 5'-monophosphoramidase HINT1

HINT1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P49773
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HINT1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.9.1.-
Processing
—
Secondary accessions
Q9H5W8
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Biological process

ApoptosisTranscriptionTranscription regulationUbl conjugation pathway

Cellular component

CytoplasmNucleus

Disease

Disease variantNeuropathy

Molecular function

HydrolaseProteaseThiol protease

Ligand

Nucleotide-binding

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