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MLH1 Antibody

CAT: 0800-R32088Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32088Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
MutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) is a protein that in humans is encoded by the MLH1 gene located on Chromosome 3. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) . It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.
CAS Number
9007-83-4
Specifications
Western blot: 0.1-0.5 µg/mL
UniProt
P40692
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids KALRSHILPPKHFTEDGNILQLANLPDLYKVFERC of human MLH1 were used as the immunogen for the MLH1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the MLH1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This MLH1 antibody is available for research use only.
Storage Conditions
After reconstitution, the MLH1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the MLH1 antibody should be determined by the researcher.
Image Legend
Western blot testing of human 1) HEK293, 2) HeLa, 3) COLO320, 4) T-47D and 5) A549 cell lysate with MLH1 antibody. Predicted molecular weight: 85/58/74 kDa (isoforms 1/2/3) .

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