CNTF Antibody

• Description :

  Ciliary neurotrophic factor (CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid (aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85% homology with the deduced aa sequence encoding human CNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. It is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.

• CAS Number :

  9007-83-4

• Specifications :

  Western blot: 0.1-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL (human protein tested) ; request BSA-free format for coating

• UniProt :

  P26441

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  Amino acids 12-200 of human CNTF were used as the immunogen for the CNTF antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, ELISA (protein)

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the CNTF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This CNTF antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the CNTF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the CNTF antibody should be determined by the researcher.

• Image Legend :

  Western blot testing of human MCF7 cell lysate with CNTF antibody. Expected/observed molecular weight ~23 kDa.