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UBA1 Antibody

CAT: 0800-R32017Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32017Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ubiquitin-like modifier activating enzyme 1 (UBA1) is an enzyme which in humans is encoded by the UBA1 gene. The protein encoded by this gene catalyzes the first step in ubiquitin conjugation, or ubiquitination, to mark cellular proteins for degradation. Specifically, UBA1 catalyzes the ATP-dependent adenylation of ubiquitin (Ub), thereby forming a thioester bond between the two. It also continues to participate in subsequent steps of ubiquination as a Ub carrier. UBA1 is one of only two human ubiquitin-activating enzymes (E1), the other being UBA6, and thus is largely responsible for protein ubiquitination in humans. Through its central role in ubiquitination, UBA1 has been linked to cell cycle regulation, endocytosis, signal transduction, apoptosis, DNA damage repair, and transcriptional regulation. Additionally, UBE1 helps regulate the NEDD8 pathway, thus implicating it in protein folding, as well as mitigating the depletion of ubiquitin levels during stress.
CAS Number
9007-83-4
Specifications
Western blot: 0.1-0.5 µg/mL
UniProt
P22314
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids HDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELN of human UBA1 were used as the immunogen for the UBA1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the UBA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This UBA1 antibody is available for research use only.
Storage Conditions
After reconstitution, the UBA1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the UBA1 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of 1) rat liver, 2) mouse liver, 3) mouse testis and 4) human HeLa lysate with UBA1 antibody. Expected molecular weight: ~118/114 kDa (isoforms a/b) .

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