CD22 Antibody

• Description:

  CD22 mediates Bcell-Bcell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. [UniProt]

• Specifications:

  Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, FACS: 1-3ug/10^6 cells

• UniProt:

  P20273

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Amino acids LAILILAICGLKLQRRWKRTQSQQGLQEN of human CD22 were used as the immunogen for the CD22 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, FACS

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

• Reconstitution:

  After reconstitution, the CD22 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This CD22 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the CD22 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the CD22 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  Western blot testing of 1) human Raji and 2) rat thymus and 3) mouse thymus lysate with CD22 antibody. Expected molecular weight: 76/95 kDa (alpha/beta, unmodified), 130-150 kDa (glycosylated) .