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PDPK1 Antibody

CAT: 0800-R31813Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31813Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
3-phosphoinositide dependent protein kinase-1 is a protein which in humans is encoded by the PDPK1 gene. PDPK1 is a master kinase, which is crucial for the activation of AKT/PKB and many other AGC kinases including PKC, S6K, SGK. An important role for PDPK1 is in the signalling pathways activated by several growth factors and hormones including insulin signaling. Mice lacking PDPK1 die during early embryonic development, indicating that this enzyme is critical for transmitting the growth-promoting signals necessary for normal mammalian development.
CAS Number
9007-83-4
Specifications
Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL
UniProt
O15530
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids YLMDPSGNAHKWCRKIQEVWRQRYQSHPDAAVQ of human PDPK1 were used as the immunogen for the PDPK1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IHC-F
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Reconstitution
After reconstitution, the PDPK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PDPK1 antibody is available for research use only.
Storage Conditions
After reconstitution, the PDPK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the PDPK1 antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Western blot testing of 1) rat liver, 2) rat lung, 3) mouse liver, 4) mouse lung, 5) human COLO320 and 6) human MCF7 lysate with PDPK1 antibody. Expected molecular weight ~63 kDa, observed here at ~70 kDa.

UniProtKB · O15530

3-phosphoinositide-dependent protein kinase 1

PDPK1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O15530
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PDPK1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
hPDK1
EC number
2.7.11.1
Processing
—
Secondary accessions
H0Y4Z0, Q59EH6, Q6FI20, Q8IV52, Q9BRD5
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoproteinUbl conjugation

Molecular function

ActivatorKinaseSerine/threonine-protein kinaseTransferase

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Cellular component

Cell junctionCell membraneCytoplasmMembraneNucleus

Biological process

TranscriptionTranscription regulation

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