SEMA3A Antibody

• Description :

  Semaphorin 3A, also called SEMA3A, SEMA1, SEMAL and COLL1, is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, SEMA3a is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of SEMA3A is associated with the progression of Alzheimers disease.

• Specifications :

  Western blot: 0.5-1 µg/mL

• UniProt :

  Q14563

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  Human partial recombinant protein (AA 550-771) was used as the immunogen for this SEMA3A antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

• Reconstitution :

  After reconstitution, the SEMA3A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This SEMA3A antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the SEMA3A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  The stated application concentrations are suggested starting amounts. Titration of the SEMA3A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot testing of SEMA3A antibody and Lane 1: 293T; 2: A549; 3: HeLa lysate. Predicted molecular weight: ~89 kDa.