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Nucleophosmin Antibody

CAT: 0800-R31754Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31754Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NPM1 or NPM (Nucleophosmin/Nucleoplasmin family member 1), also known as Nucleolar phosphoprotein B23 or numatrin, is a protein that in humans is encoded by the NPM1 gene. Chan et al. (1989) found that NPM is a nucleolar phosphoprotein that is more abundant in tumor cells than in normal resting cells. Stimulation of the growth of normal cells, e.g., mitogen activation of B lymphocytes, was accompanied by an increase in Nucleophosmin protein level. They stated that the protein is likely involved in the assembly of ribosomal proteins into ribosomes. Electron microscopic study indicated that Nucleophosmin is concentrated in the granular region of the nucleolus, where ribosome assembly occurs.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Human partial recombinant protein (AA 1-294) was used as the immunogen for this Nucleophosmin antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the Nucleophosmin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Nucleophosmin antibody is available for research use only.
Storage Conditions
After reconstitution, the Nucleophosmin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Nucleophosmin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nucleus, Cytoplasm
Image Legend
Immunofluorescent staining of FFPE human intestinal cancer tissue with Nucleophosmin antibody. HIER: steam section in pH8 EDTA for 20 min.

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