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Calpain Antibody

CAT: 0800-R31743Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31743Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Calpain 1 is also known as CAPN, CAPN1 and muCL. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. The CPN1 gene encodes the catalytic subunit of the ubiquitous enzyme, Calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Human recombinant protein from the CAPN1 catalytic subunit (AA 396-555) was used as the immunogen for this Calpain antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the Calpain antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Calpain antibody is available for research use only.
Storage Conditions
After reconstitution, the Calpain antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Calpain antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Immunofluorescent staining of FFPE human U-2 OS cells with Calpain antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.
Product Datasheet
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