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MGMT Antibody

CAT: 0800-R31679Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31679Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
O6-alkylguanine DNA alkyltransferase (also known as AGT, MGMT or AGAT) is a protein that in humans is encoded by the MGMT gene. This gene is crucial for genome stability. The protein repairs the naturally occurring mutagenic DNA lesion O6-methylguanine back to guanine and prevents mismatch and errors during DNA replication and transcription. It has been found that methylation of the MGMT promoter in the tumor was associated with longer survival.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunocytochemistry: 1-2 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
Host
Rabbit
Reactivity
Human
Immunogen
Human partial recombinant protein (AA 2-207) was used as the immunogen for this MGMT antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ICC, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the MGMT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This MGMT antibody is available for research use only.
Storage Conditions
After reconstitution, the MGMT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the MGMT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Immunofluorescent staining of FFPE human MCF7 cells with MGMT antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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