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P300 Antibody (KAT3B)

CAT: 0800-R31592Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31592Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
E1A binding protein p300 is a protein that in humans is encoded by the EP300 gene. The gene is located on the long (q) arm of the human chromosome 22 at position 13.2. This protein regulates the activity of many genes in tissues throughout the body. It plays an essential role in regulating cell growth and division, prompting cells to mature and assume specialized functions (differentiate), and preventing the growth of cancerous tumors. p300 appears to be critical for normal development before and after birth. It carries out its function by activating transcription. In addition, the protein functions as histone acetyltransferase that regulates transcription via chromatin remodeling, and is important in the processes of cell proliferation and differentiation. p300 also mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Human partial recombinant protein (AA 2065-2414) was used as the immunogen for this KAT3B / p300 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the p300 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This p300 antibody is available for research use only.
Storage Conditions
After reconstitution, the p300 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the p300 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Intracellular
Image Legend
Western blot testing of 1) human COLO-320, 2) rat PC-12 and 3) mouse NIH 3T3 cell lysate with p300 antibody. Predicted molecular weight ~300 kDa.

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