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ATF1 Antibody

CAT: 0800-R31551Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31551Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Activating transcription factor 1 is a protein that in humans is encoded by the ATF1 gene. This gene encodes an activating transcription factor which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. It is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3) . Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P18846
Host
Rabbit
Reactivity
Human
Immunogen
Human partial recombinant protein (AA 1-271) was used as the immunogen for this ATF1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the ATF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ATF1 antibody is available for research use only.
Storage Conditions
After reconstitution, the ATF1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting points. Titration of the ATF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
Flow cytometry testing of human SiHa cells with ATF1 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= ATF1 antibody.

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