CD45 Antibody

• Description:

  Cluster of Differentiation 45, also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene. It is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase. Targeted disruption of the gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, it negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. It has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be fine-tuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of N-linked carbohydrates.

• Specifications:

  Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 2-4 µg/mL

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Human partial recombinant protein (AA 1113-1304) was used as the immunogen for this CD45 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, FACS, IF

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the CD45 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This CD45 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the CD45 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the CD45 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, membrane

• Image Legend:

  Western blot testing of CD45 antibody and Lane 1: Jurkat; 2: Raji lysate. Expected molecular weight: ~147/180-220 kDa (unmodified/glycosylated) .