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VWF Antibody

CAT: 0800-R31491Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31491Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Von Willebrand factor (VWF) is a blood glycoprotein involved in hemostasis. It is mapped to 12p13.31. The gene encodes a large multimeric glycoprotein that plays a central role in the blood coagulation system, serving both as a major mediator of platelet-vessel wall interaction and platelet adhesion, and as a carrier for coagulation factor VIII. VWF released from endothelial cell Weibel-Palade bodies bound particularly avidly to the extracellular matrix. VWF deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells
Host
Rabbit
Reactivity
Human
Immunogen
Human partial recombinant protein (AA 2535-2813) was used as the immunogen for this VWF antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IHC-F, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Reconstitution
After reconstitution, the VWF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This VWF antibody is available for research use only.
Storage Conditions
After reconstitution, the VWF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the VWF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic
Image Legend
Western blot testing of VWF antibody and human HT1080 lysate. Predicted molecular weight ~309 kDa.

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