Calbindin Antibody / CALB1

• Description:

  Calbindin is a calcium-binding protein belonging to the troponin C superfamily. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunoprecipitation: 2ug per 500ug of lysate

• UniProt:

  P05937

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Human partial recombinant protein (AA 2-175) was used as the immunogen for this Calbindin antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, IP

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the Calbindin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Calbindin antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Calbindin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the Calbindin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, nuclear, secreted

• Image Legend:

  IHC staining of FFPE mouse kidney tissue with Calbindin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.