CNTF Antibody

• Description:

  Ciliary neurotrophic factor (CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The mouse gene is on chromosome 19 and that its expression is unaffected in the mouse neurologic mutant wobbler, a form of spinal muscular atrophy. The protein is highly conserved in evolution. The protein is a potent survival factor for neurons and oligodendrocytes, and it may be involved in reducing tissue destruction during inflammatory attacks. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.

• Specifications:

  Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  Mouse partial recombinant protein (AA 2-198) was used as the immunogen for this CNTF antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the CNTF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This CNTF antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the CNTF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the CNTF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of 1) rat brain and 2) mouse brain with CNTF antibody. Predicted molecular weight ~23 kDa.