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Ki67 Antibody

CAT: 0800-R31458Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31458Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Proliferation-related Ki-67 antigen, also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL
Host
Rabbit
Reactivity
Human
Immunogen
Human partial recombinant protein (AA 2860-3256) was used as the immunogen for this Ki67 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ICC
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the Ki67 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Ki67 antibody is available for research use only.
Storage Conditions
After reconstitution, the Ki67 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Ki67 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
Western blot testing of Ki67 antibody and human samples 1: HeLa; 2: MCF-7; 3: COLO320; 4: HEPG2; 5: SKOV lysate. Predicted molecular weight ~350 kDa.

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