Huntingtin Antibody / HTT

• Description:

  The Huntingtin gene, also called HTT or HD (Huntington disease), is the IT15 (interesting transcript 15) gene which codes for a protein called the Huntingtin. The protein has no sequence homology with other proteins and is highly expressed in neurons and tests in humans and rodents. HTT upregulates the expression of Brain Derived Neurotrophic Factor (BDNF) at the transcription level, and this gene is required for normal development. HTT is involved in vesicle trafficking as it interacts with HIP1, a clathrin-binding protein, to mediate endocytosis, the absorption of materials into a cell. HTT was also visualized as punctate staining likely to represent nerve endings. Additionally, wildtype Huntingtin may function in the nucleus in the assembly of nuclear matrix-bound protein complexes involved with transcriptional repression and RNA processing.

• Specifications:

  Western blot: 0.5-1 µg/mL

• UniProt:

  P42858

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  An amino acid sequence from the C-terminus of human HTT (ELDRRAFQSVLEVVAA) was used as the immunogen for this Huntingtin antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution:

  After reconstitution, the Huntingtin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Huntingtin antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Huntingtin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the Huntingtin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of Huntingtin antibody and Lane 1: human HeLa cell lysate and 2: human U-87 MG cell lysate. Predicted molecular weight: ~350 kDa.