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UBC9 Antibody

CAT: 0800-R31382Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31382Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SUMO-conjugating enzyme UBC9, also called UBE2I, is a protein that in humans is encoded by the UBE2I gene. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. UBE2I could fully complement the mutant phenotype of a yeast ubc9 mutant strain. This gene may play a similar role via interaction with WT1, which is able to impose a block to cell cycle progression in eukaryotic cells. Additionally, it could support the growth of yeast ubc9 temperature-sensitive mutants at nonpermissive temperatures, indicating that the gene is a functional homolog of yeast ubc9. The protein is specifically associated with FHIT, such as FHIT may be involved in cell cycle control through its interaction with UBE2I.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
P63279
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the C-terminus of human UBC9/UBE2I (IKQILLGIQELLNE) was used as the immunogen for this UBC9 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the UBC9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This UBC9 antibody is available for research use only.
Storage Conditions
After reconstitution, the UBC9 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the UBC9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of 1) human placenta, 2) human K562, 3) human HepG2, 4) rat brain and 5) rat kidney tissue lysate with UBC9 antibody. Predicted molecular weight ~18 kDa.

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