GPR30 Antibody

• Description :

  G-protein coupled estrogen receptor 1 (GPER1), also known as the GPR30 or CEPR, is a G protein-coupled receptor that in humans is encode d by the GPER gene. This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. GPR30 therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized.

• Specifications :

  Western blot: 0.5-1 µg/mL

• UniProt :

  Q99527

• Host :

  Rabbit

• Reactivity :

  Human, Monkey

• Immunogen :

  An amino acid sequence from the C-terminus of human G-protein coupled receptor 30 (ENVFISVHLLQRTQ) was used as the immunogen for this GPR30 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution :

  After reconstitution, the GPR30 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This GPR30 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the GPR30 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  The stated application concentrations are suggested starting amounts. Titration of the GPR30 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot testing of GPR30 antibody and Lane 1: human COLO320; 2: human MCF-7; 3: monkey COS7 cell lysate. Expected molecular weight: 40-60 kDa depending on glycosylation level.