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RGS14 Antibody

CAT: 0800-R31283Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31283Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Regulator of G-protein signaling 14 is a protein that in humans is encoded by the RGS14 gene. This gene encodes a member of the regulator of G-protein signaling family. By genomic sequence analysis, this gene is mapped to chromosome 5q35.3. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
O43566
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the C-terminus of human Regulator of G-protein signaling 14 (CTVLADQEVRLENR) was used as the immunogen for this RGS14 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the RGS14 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This RGS14 antibody is available for research use only.
Storage Conditions
After reconstitution, the RGS14 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the RGS14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of RGS14 antibody and Lane 1: rat thymus; 2: (r) spleen; 3: human Raji; 4: (h) CEM; 5: (h) Jurkat cell lysate. Expected/observed size ~65KD
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