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VCP Antibody

CAT: 0800-R31254Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31254Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Valosin-containing protein, also called Cdc48, is an enzyme that in humans is encoded by the VCP gene. It is a member of the AAA+ (ATPase associated with various activities) protein family. The gene maps to chromosome 9p13.3. It is necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. It is involved in the formation of the transitional endoplasmic reticulum. This gene plays a role in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunohistochemistry (Frozen) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P55072
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
An amino acid sequence from the C-terminus of human Valosin-containing protein (DNDIRKYEMFAQTLQQSR) was used as the immunogen for this VCP antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IHC-F, ICC, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the VCP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This VCP antibody is available for research use only.
Storage Conditions
After reconstitution, the VCP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the VCP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of VCP antibody and rat samples 1: brain; 2: kidney; 3: liver; 4: lung; and human samples 5: HeLa; 6: HL-60; 7: A431; 8: A549; 9: SMMC-7721 cell lysate. Predicted/observed molecular weight: ~89/97 kDa.
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