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SMAD1 Antibody

CAT: 0800-R31227Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31227Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mothers against decapentaplegic homolog 1, also known as SMAD family member 1, is a protein that in humans is encoded by the SMAD1 gene. SMAD1 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. The gene was assigned to human chromosome 4q31.21. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL
UniProt
P70340
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the C-terminus of mouse SMAD family member 1 (AEISRGDVQAVAYEE) was used as the immunogen for this Smad1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ICC
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the SMAD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This SMAD1 antibody is available for research use only.
Storage Conditions
After reconstitution, the SMAD1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the SMAD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of SMAD1 antibody and Lane 1: SMMC-7721; 2: K562; 3: HT1080; 4: HeLa; 5: Jurkat cell lysate. Predicted molecular weight: 52~60 kDa.
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