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PKM2 Antibody

CAT: 0800-R31156Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31156Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Pyruvate Kinase, Muscle, also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2) . By in situ hybridization, Popescu and Cheng (1990) mapped the gene to 15q24-q25. Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
P14618
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the N-terminus of human PKM2 (SHGTHEYHAETIKNV) was used as the immunogen for this PKM2 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the PKM2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PKM2 antibody is available for research use only.
Storage Conditions
After reconstitution, the PKM2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the PKM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of PKM2 antibody and Lane 1: HeLa; 2: MCF-7; 3: A549 cell lysate. Predicted molecular weight ~58 KDa.
Product Datasheet
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