Products for Research Use Only

VEGFR2 Antibody

CAT: 0800-R31095Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31095Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
KDR (Kinase Insert Domain Receptor), also known as FLK1, VEGFR 2 or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Sait et al. (1995) likewise corrected the assignment to chromosome 4q11-q12 to the same region occupied also by PDGFRA and KIT, thus indicating the location of a cluster of receptor tyrosine kinase genes. Vascular endothelial growth factor (VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1 (Flk1) in mouse, is exclusively expressed in endothelial cells (Plate et al., 1993) . Flk1 is the mouse homolog of KDR (Matthews et al., 1991) .
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunohistochemistry (Frozen) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL
UniProt
P35968
Host
Rabbit
Reactivity
Human
Immunogen
An amino acid sequence from the middle region of human VEGF Receptor 2 (HHIHWYWQLEEECANE) was used as the immunogen for this VEGFR2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IHC-F, ICC
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the VEGFR2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This VEGFR2 antibody is available for research use only.
Storage Conditions
After reconstitution, the VEGFR2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting points. Titration of the VEGFR2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic and cell surface
Image Legend
Western blot testing with VEGFR2 antibody and SMMC-7721 lysate. Predicted molecular weight: ~152 (immature), 180-200 kDa (intermediate) and 220-230 kDa (mature) .

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