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VGF Antibody

CAT: 0800-R31090Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31090Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
VGF, also called NERP-2 (Neuroendocrine regulatory peptide-2), is a protein and neuropeptide that may play a role in regulating energy homeostasis, metabolism. Canu et al. (1997) noted that rat Vgf encodes a predicted 70-kD polypeptide that shares similarities with the secretogranin/chromogranin family and is found in the secretory granules of subsets of neurons and endocrine cells. By fluorescence in situ hybridization, Canu et al. (1997) assigned the VGF gene to 7q22. Canu et al. (1997) demonstrated that the single-copy human gene spans 6 kb of genomic DNA and contains 2 exons. The entire protein is encoded by exon 2, while exon 1 contains only 5-prime untranslated sequence. The structural organization of the human gene is similar to that described for the rat gene (Salton et al., 1991), and both the translated and the untranslated regions show a high degree of sequence homology to the rat gene.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
O15240
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the C-terminus of human VGF (QARRAQEEAEAEERRLQEQ) was used as the immunogen for this VGF antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the VGF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This VGF antibody is available for research use only.
Storage Conditions
After reconstitution, the VGF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the VGF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of VGF antibody and Lane 1: human U87; 2: (h) SHG-44; 3: mouse Neuro-2a cell lysate. Expected molecular weight: ~90/70kDa (unprocessed/processed) .

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