Products for Research Use Only
RegisterLogin

DNA-PKcs Antibody

CAT: 0800-R31076Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-R31076Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Protein Kinase DNA-Activated catalytic subunit, also called DNAPK, HYRC1, p350 or DNPK1, is an enzyme that in humans is encoded by the PRKDC gene. DNA-PKcs belongs to the phosphatidylinositol 3-kinase-related kinase protein family. Satoh et al. (1997) mapped the MCM4 gene to 8q11.2 by FISH. Based on the close proximity of the PRKDC and MCM4 genes, it was assumed that the PRKDC gene also maps to this location. Anderson and Lees-Miller (1992) noted that DNA-PKcs had been shown in vitro to phosphorylate several transcription factors, suggesting that it functions in cell homeostasis by modulating transcription. Daniel et al. (1999) demonstrated that the DNA-PKcs protein participates in retroviral DNA integration, which is catalyzed by the viral protein integrase.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
P78527
Host
Rabbit
Reactivity
Human
Immunogen
An amino acid sequence from the N-terminus of human PRKDC (AKNAEMHKNKLQYFMEQFY) was used as the immunogen for this DNA-PKcs antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the DNA-PKcs antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This DNA-PKcs antibody is available for research use only.
Storage Conditions
After reconstitution, the DNA-PKcs antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the DNA-PKcs antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of DNA PKcs antibody and HeLa cell lysate. Predicted/observed size ~460KD

UniProtKB · P78527

DNA-dependent protein kinase catalytic subunit

PRKDC_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P78527
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PRKDC
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
DNA-PK catalytic subunit, DNA-PKcs
EC number
2.7.11.1
Processing
—
Secondary accessions
P78528, Q13327, Q13337, Q14175, Q59H99, Q7Z611, Q96SE6, Q9UME3
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Biological process

Biological rhythmsDNA damageDNA recombinationDNA repairImmunityInnate immunityRibosome biogenesis

Cellular component

CytoplasmNucleus

Disease

Disease variantSCID

Molecular function

DNA-bindingKinaseSerine/threonine-protein kinaseTransferase

Domain

RepeatTPR repeat
Product Datasheet
Loading...

Related Products

CatalogName
V8536-20UGTdT Antibody / DNA nucleotidylexotransferase
RQ5034TdT Antibody / DNA nucleotidylexotransferase
V3104-100UGDouble Stranded DNA Antibody
V8399SAF-100UGTdT Antibody / DNA nucleotidylexotransferase
F41538-0.08MLTdT Antibody / DNA nucleotidylexotransferase
V8399-20UGTdT Antibody / DNA nucleotidylexotransferase
V5831-20UGDNA-RNA Hybrid Antibody
V5831SAF-100UGDNA-RNA Hybrid Antibody
V8879SAF-100UGDNA nucleotidylexotransferase Antibody / TdT
V8399-100UGTdT Antibody / DNA nucleotidylexotransferase

Alternative Products

CatalogName
20R-2346DNA PKcs antibody
C0174-01DNA-PKCS Antibody
313732-01DNA-PKCS
E8ET1610-12DNA PKcs

Popular Products