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LCAT Antibody

CAT: 0800-R31073Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31073Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
LCAT (Lecithin: Cholesterol Acyltransferase), is an enzyme that converts free cholesterol into cholesteryl ester. Azoulay et al. (1987) used a cDNA clone corresponding to LCAT to assign the locus to 16q22 through the analysis of DNA from somatic cell hybrids and in situ hybridization. LCAT plays an important role in lipoprotein metabolism, especially in the process termed reverse cholesterol transport The enzyme is synthesized in the liver and circulates in blood plasma as a complex with components of high density lipoprotein (HDL) . Cholesterol from peripheral cells is transferred to HDL particles, esterified through the action of LCAT on HDL, and incorporated into the core of the lipoprotein. The cholesterol ester is thereby transported to the liver (Jonas, 2000) .
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
P04180
Host
Rabbit
Reactivity
Human, Rat
Immunogen
Amino acids 300-313 (RDFQRFFADLHFEE-human) were used as the immunogen for this LCAT antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the LCAT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This LCAT antibody is available for research use only.
Storage Conditions
After reconstitution, the LCAT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the LCAT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of LCAT antibody and Lane 1: rat brain; 2: U87; 3: HeLa; 4: SMMC-7721 cell lysate. Observed size 50~75KD, depending on glycosylation level.

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