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RIAM Antibody

CAT: 0800-R31063Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31063Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
RIAM (Rap1-interacting adaptor molecule), also called RARP1 and APBB1-Interacting Protein, is a protein that in humans is encoded by the APBB1IP gene. By genomic sequence analysis, Lafuente et al. (2004) mapped the gene to chromosome 10p12.1. Using promoter-reporter gene assays, Inagaki et al. (2003) found that RIAM suppressed transcription from AP1 and SRE sites, but not CRE sites, in all cell lines examined. The proline-rich regions of RIAM suppressed AP1 transactivation. Lafuente et al. (2004) found that the protein interacted with profilin and VASP, molecules that regulate actin dynamics, as well as with RAP1-GTP.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunohistochemistry (Frozen) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
Q7Z5R6
Host
Rabbit
Reactivity
Human, Rat
Immunogen
An amino acid sequence from the C-terminus of human Rap1-interacting adaptor molecule (EQDFMSDLMKALQKKRGNVS) was used as the immunogen for this RIAM antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IHC-F, ICC, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the RIAM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This RIAM antibody is available for research use only.
Storage Conditions
After reconstitution, the RIAM antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the RIAM antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of RIAM antibody and human 1) ThP-1, 2) Jurkat, 3) Raji, 4) U937, 5) rat thymus and 6) mouse thymus lysate. Predicted molecular weight ~73~110 kDa.

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